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Jena Bioscience:dsRNA抗體

發布者:艾美捷科技    發布時間:2024-03-11     
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雙鏈(ds)RNA的形成是病毒感染的標志,對于誘導先天免疫至關重要。dsRNA還參與基因沉默,并在體外轉錄基因合成過程中作為副產物產生??筪sRNA單克隆抗體是在細胞培養和組織中檢測dsRNA的有效工具,主要應用于如下領域:  


dsRNA.png


1.用于表征和檢測具有dsRNA基因組或中間體的病毒(包括SARS,丙型肝炎,登革熱或西尼羅河病毒)。

2.作為診斷工具,用于確定未知病原體是病毒還是細菌來源

3.用于體外轉錄(m)RNA制備的質量控制 


dsRNA抗體精選與推薦:

貨號產品描述選擇指南
RNT-SCI-10010500抗 dsRNA 單克隆抗體 J2小鼠,IgG2a,κ 輕鏈IVT體外轉錄質控,金標準;文章最多
RNT-SCI-10040500抗 dsRNA 單克隆抗體 J5小鼠,IgG2b,κ 輕鏈更適合IF實驗
RNT-SCI-10020500抗 dsRNA 單克隆抗體 K1小鼠,IgG2a,κ 輕鏈更適合 Poly I:C 檢測
RNT-SCI-10050100抗 dsRNA ,抗體套裝J2、J5 和 K1 抗體的尺寸樣本推薦用于 J2、J5 和 K1 抗體的比較測試
RNT-SCI-10030010抗 dsRNA 單克隆抗體 K2小鼠,IgM,κ 輕鏈更適合ELISA
RNT-SCI-10080100dsRNA 142 bp合成 dsRNA陽性對照

dsRNA-1.png

Fig.1:A)使用抗雙鏈RNA單克隆抗體J2在SARS-CoV感染的Vero細胞中檢測dsRNA的免疫熒光。 1:未感染的細胞。 標尺:20微米(根據[4]進行修改)。 B)使用抗雙鏈RNA單克隆抗體J2在SARS-Cov感染的Vero E6細胞的雙膜囊泡(DMVs)中進行免疫電子顯微鏡檢測(根據[5]進行修改)。 C)使用抗雙鏈RNA單克隆抗體J2在柯薩奇病毒感染的新生小鼠FFPE組織中檢測dsRNA。 1:小鼠心臟,2:褐色脂肪,3:胰腺,4:唾液腺,5:中樞神經系統神經節,6:未感染組織。  


《Cell文章推薦》:

SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9,Cell  |  2023 Oct 26

Article Snippet

".. washed once with ice-cold PBS, followed by two washes with PBS at room temperature.. For combining immunostaining with HCR-based detection, we used anti-J2 antibody (Jena BioscienceRNT-SCI-10010200, 1:500) or anti-SND1 antibody (Proteintech, 60265-1- Ig, 1:500).. Incubation with the primary antibody was performed for 1 h at room temperature.Incubation with .."

dsRNA-2.png


Figure Legend

"... (B) Representative images of IF staining for dsRNA (J2 antibody) using HCR detection in SARS-CoV-2 infected"   


更多歷史參考文獻: 

 [1] Sch?nborn et al. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19: 2993. 

 [2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods47: 255. 

 [3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford 

 [4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80(10): 5059. 

 [5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6(9): e226.

 [6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 180. 

 [7] Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research 39(21): e142.



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