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Cytoskeleton小G蛋白&細胞成像產品線新春大促啦!

發布者:艾美捷科技    發布時間:2024-01-12     
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為答謝和回饋廣大科研工作者們長期以來對武漢艾美捷科技有限公司的大力支持,在新春到來之際,艾美捷科技聯合細胞骨架領域頂級供應商 Cytoskeleton 聯合推出:小G蛋白&細胞成像產品線新春超值大促,一年僅此一次,千萬不要錯過??!本次促銷活動產品涵蓋  小G蛋白活化檢測試劑盒,激動劑、Tubulin,Actin,溶酶體,DNA,質膜等細胞組分成像的熒光染料等。 


活動詳情:

時間:2024年1月15日-2024年2月28日

對象:僅限中國區終端客戶

品目:Cytoskeleton小G蛋白&細胞成像相關產品(詳情見下方活動品目列表)

內容:直享 6.5折超低優惠! 


活動品目列表:

分類貨號產品名稱規格原價折后價
Actin抗體CSK-AAN02Anti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1)1x500ul4547 2955
CSK-AAN02-SAnti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1)1x125ul1634 1062
小G蛋白活化檢測試劑盒CSK-BK001Actin Binding Protein Spin-Down Assay KitKIT30-100assays12793 8315
CSK-BK003Actin Polymerization Biochem KitKIT30-100assays14581 9477
CSK-BK008Ras Activation Assay Biochem KitKIT50assays12346 8025
CSK-BK008-SRas Activation Assay Biochem KitKIT20assays6073 3947
CSK-BK030Combo RhoA/Rac1/Cdc42 Activation Assay KitKIT3x10assays16276 10579
CSK-BK032-SArf1 Pulldown Activation Assay KitKIT20assays8015 5210
CSK-BK033-SArf6 Pulldown Activation Assay KitKIT20assays8015 5210
CSK-BK034Cdc42 Pulldown Activation Assay KitKIT50assays13193 8576
CSK-BK034-SCdc42 Pulldown Activation Assay KitKIT20assays6073 3947
CSK-BK035Rac1 Pulldown Activation Assay KitKIT50assays13193 8576
CSK-BK035-SRac1 Pulldown Activation Assay KitKIT20assays6073 3947
CSK-BK036RhoA Pulldown Activation Assay KitKIT80assays13193 8576
CSK-BK036-SRhoA Pulldown Activation Assay KitKIT20assays6073 3947
CSK-BK037G-Actin : F-Actin In Vivo Assay KitKIT30-100assays12407 8065
CSK-BK121RhoA G-LISA Activation Assay (luminescence)KIT96assays15367 9988
CSK-BK124RhoA G-LISA Activation Assay (colorimetric)KIT96assays15367 9988
CSK-BK124-SRhoA G-LISA Activation Assay (colorimetric)KIT24assays9279 6031
CSK-BK124-XLRhoA G-LISA Activation Assay (colorimetric)LargeQuantities詢價詢價
CSK-BK125Rac1,2,3 G-LISA Activation Assay Kit (colorimetric)KIT96assays15367 9988
CSK-BK126Rac1 G-LISA Activation Assay KitKIT96assays15367 9988
CSK-BK127Cdc42 G-LISA Activation Assay Kit (colorimetric)KIT96assays15367 9988
CSK-BK127-SCdc42 G-LISA Activation Assay Kit (colorimetric)KIT24assays9279 6031
CSK-BK128Rac1 G-LISA Activation Assay Kit (colorimetric)KIT96assays15367 9988
CSK-BK128-SRac1 G-LISA Activation Assay Kit (colorimetric)KIT24assays9279 6031
CSK-BK129RalA G-LISA Activation Assay Kit (colorimetric)KIT96assays15367 9988
CSK-BK131Ras G-LISA Activation Assay Kit (colorimetric)KIT96assays15367 9988
CSK-BK135G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) (colorimetric)3KITS(24assays/kit)27342 17772
CSK-BK150RhoA ELISA Kit (colorimetric)KIT96assays8385 5450
小G蛋白相關活化劑及抑制劑CSK-CN01-ARho Activator5x10units3514 2284
CSK-CN01-BRho Activator20x10units12793 8315
CSK-CN02-ARac and Cdc42 Activator5x10units3514 2284
CSK-CN02-BRac and Cdc42 Activator20x10units12793 8315
CSK-CN03-ARho activator II3x20ug3514 2284
CSK-CN03-BRho activator II9x20ug9895 6432
CSK-CN03-XLRho activator IILargeQuantities詢價詢價
CSK-CN04-ARho/Rac/Cdc42 Activator I3x20ug3514 2284
CSK-CN04-BRho/Rac/Cdc42 Activator I9x20ug9895 6432
CSK-CT03-AC3 transferase protein1x25ug3406 2214
CSK-CT03-CC3 transferase protein4x25ug12407 8065
CSK-CT04-ACell Permeable Rho Inhibitor (C3 Trans based))1x20ug3406 2214
CSK-CT04-BCell Permeable Rho Inhibitor (C3 Trans based))5x20ug11251 7313
CSK-CT04-CCell Permeable Rho Inhibitor (C3 Trans based))20x20ug27712 18013
Tubulin,Actin,溶酶體,DNA,質膜染料CSK-CY-SC001SiR-Actin Kit50-300slides9309 6051
CSK-CY-SC002SiR-Tubulin Kit50-300slides9309 6051
CSK-CY-SC007SiR-DNA Kit50-300slides5996 3897
CSK-CY-SC012SiR-Lysosome Kit50-300slides6720 4368
CSK-CY-SC101SPY505-DNA Probe100slides5996 3897
CSK-CY-SC201SPY555-DNA Probe100slides5996 3897
CSK-CY-SC501SPY650-DNA Probe100slides5996 3897
CSK-MG01-02MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499  em 507  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3884 2525
CSK-MG01-10MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499  em 507  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides7306 4749
CSK-MG02-02MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555  em 570  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3884 2525
CSK-MG02-10MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555  em 570  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides7306 4749
CSK-MG03-02MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580  em 620 50-300 slides  (MEMBRIGHT Family Probe)50-300slides3884 2525
CSK-MG03-10MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580  em 620  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides7306 4749
CSK-MG04-02MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650  em 673  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3884 2525
CSK-MG04-10MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650  em 673  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides7306 4749
CSK-MG05-02MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650  em 720  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3884 2525
CSK-MG05-10MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650  em 720  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides7306 4749
CSK-MG06MemGlow? NR4A Membrane Polarity Probe ex 553  em 637  50-300 slides  (MEMBRIGHT Family Probe)50-300slides7306 4749
CSK-MG07MemGlow? NR12A Membrane Polarity Probe ex 555  em 637  100-600 slides  (MEMBRIGHT Family Probe)100-600slides7306 4749
CSK-MG08MemGlow? NR12 S Membrane Polarity Probe ex 550  em 633  100-600 slides  (MEMBRIGHT Family Probe)100-600slides7306 4749
CSK-MG11LipiBright? 530 SMCy3.5: Fluorogenic Lipid Droplet Probe ex 530  em 558 70 nmoles  (MEMBRIGHT Family Probe)70nmoles5949 3867
CSK-MG12LipiBright? 650 SMCy5.5: Fluorogenic Lipid Droplet Probe ex 650  em 670 70 nmoles  (MEMBRIGHT Family Probe)70nmoles5949 3867
CSK-MG13LipiBright? 700 SMCy7.0: Fluorogenic Lipid Droplet Probe ex 700  em 770 70 nmoles  (MEMBRIGHT Family Probe)70nmoles5949 3867
CSK-PHDG1-AActi-stain? 488 phalloidin1x500ul(300slides)3684 2394
CSK-PHDH1-AActi-stain? 555 phalloidin1x500ul(300slides)3684 2394
CSK-PHDN1-AActi-stain? 670 phalloidin1x500ul(300slides)3684 2394
CSK-PHDR1Rhodamine Phalloidin (14 ?M in methanol)1x500ul3684 2394

*本活動最終解釋權歸艾美捷科技所有 


產品應用詳情了解:

1.小G蛋白活化檢測:

小G蛋白(Small G Protein)因分子量只有20-30KD而得名,同時具有GTP酶活性,在細胞信號通路中發揮分子開關的功能,參與調控很多生物學過程,包括分泌活動和細胞骨架的裝配等。目前發現的小G蛋白包括 Rho, Rac, Arf1 & 6, Ras, Ral 等。小G蛋白的共同特點是,當結合了GTP時即成為活化形式,這時可作用于下游分子使之活化,而當GTP水解成為GDP時(自身為GTP酶)則恢復到非活化狀態。    


Pull-down和G-LISA實驗都被廣泛地用于研究小G蛋白的活性。Pull-down實驗又叫蛋白質體外結合實驗,可用于測定活性的GTPase亞型,可作為對G-LISA實驗的補充。G-LISA 實驗是一種改進的ELISA,可測定3-D細胞系、原代細胞裂解液及組織裂解液中有活性的GTPase,使GTPase定量的準確性增加。  


Pull-down及G-LISA實驗結果展示:

Small G Protein-1.png

Lanes 4 & 5:10 ng/ ml of EGF, 2min

Lanes 2 & 3: Persistent serum starvation

Lanes 1: 20 ng of recombinant Rac1-His protein run as a western blot standard

Small G Protein-2.png

分別用CN01(藍色Activators)和LPA(紅色)處理后RhoA的活化進程


2.細胞組分成像結果展示:

SiR-Tubulin.png

Hela細胞有絲分裂,SiR-Tubulin染色Tubulin蛋白(綠色)

SiR700 Actin.png

SiR和SiR700 Actin染色活細胞的Actin

F-Actin.png

鬼筆環肽染色固定細胞F-Actin

HeLa.png

HeLa細胞有絲分裂延時成像,有絲分裂是一個高度敏感的過程,顯示SiR-DNA染料的低細胞毒性。


Cytoskeleton公司成立于1993年,專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發與生產。公司提供藥物篩選、信號轉導、蛋白質轉錄后修飾(PTM)、細胞骨架研究相關的系列試劑盒和產品,尤其以細胞骨架相關研究見長,既能滿足于樣品較少的科學研究,也可以用于小規模篩選研究和高通量大規模篩選研究。   


艾美捷科技與國內外優秀的生物試劑供應商優保持著密切的合作關系,目前已成為眾多國際知名品牌的中國總代理或一級代理,主要包括:AAT Bioquest、Abbexa、Abnova、Advanced Targeting Systems、Agrisera、Atlas Antibodies、BellBrook、Bio-Helix、Biomatik、Biosensis、BioVendor、CalBioreagents、Cayman Chem、Cell Biolabs、Columbia Biosciences、Crystal Chem、Cytoskeleton、DIAsource、Duchefa、Ebba Biotech、Echelon Biosciences、ECM Biosciences、Enzo Life Sciences、Epigentek、Equitech-Bio、FabGennix、G-Biosciences、GeneCopoeia、Hycult Biotech、ichorbio、Icosagen、Immunochemistry、Immundiagnostik、ImmunoReagents、IQ Products、Jackson、Kamiya Biomedical、LC Labs、LifeSensors、Lumiprobe、Mabtech、Matreya、Medkoo Biosciences、MyBioSource、Nanoprobe、Norgen Biotek、ProSci、ProSpec、ReliaTech、Rockland、SouthernBiotech、StressMarq、SySy、TRC、US Biological、Worthington 等,可以在短時間內為用戶提供專業的前沿資訊、完備的產品及物流服務。   


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